Start Date: Monday June 3rd -----------------------------------> End Date: ~ Thursday August 1st Journal Club/Group Meeting - once a week Location: GEA 100 (Gearing Hall)
We are going to work on our targets that we started picking in the Target Discovery Exercise.
First, we will review those and see if they are good.
Then we will do some practice cloning procedures to teach you guys the new DNA cloning skills.
Then we will proceed to work on cloning our targets
Then we will express, purify and characterize our targets.
Store reagents at the proper temperature & in the right location (e.g. correct freezer box)
Label all reagents (tubes, boxes, liguids, powders) with VDS and Date (also include your initials if it is yours)
Write down everything that you do in lab into your lab notebook
Clean your area when done and return reagents and materials to their 'homes'
SCHEDULES
please enter your summer class schedule here (if you are also taking classes in the summer):https://docs.google.com/spreadsheet/ccc?key=0AvGl3lMyhDsodDBPVkpsWDNReV9MQ25DSkpzYkJ6X2c&usp=sharing
Start Date: Monday June 3rd -----------------------------------> End Date: ~ Thursday August 1st
Journal Club/Group Meeting - once a week
Location: GEA 100 (Gearing Hall)
Journal Club Summ13
The Plan
We are going to work on our targets that we started picking in the Target Discovery Exercise.First, we will review those and see if they are good.
Then we will do some practice cloning procedures to teach you guys the new DNA cloning skills.
Then we will proceed to work on cloning our targets
Then we will express, purify and characterize our targets.
Skills List:
Target DiscoveryPCR (primer overlap)
Restriction Enzyme Digest
Cloning
Plasmid Midi-Prep
DNA Sequencing
Protein Expression, Purification, Characterization (Large scale)
FPLC
Enzyme assays
Things to Keep in Mind
Store reagents at the proper temperature & in the right location (e.g. correct freezer box)Label all reagents (tubes, boxes, liguids, powders) with VDS and Date (also include your initials if it is yours)
Write down everything that you do in lab into your lab notebook
Clean your area when done and return reagents and materials to their 'homes'
Summer Researchers:
Mentors:Daniel, Michael, Andrew, Ruifei
OLD
SummerResearchFormVDS_2013
https://docs.google.com/forms/d/1lB_xT7y7c-R0m5DKy08ItI3I0tJB-0qD6cfxw1-_mgQ/viewform#start=invite